De Novo Gold Nanoparticles Activate P53 by Inhibiting NF-Kb Signalling in Breast Cancer Cells

Abstract

The aim of this study was to make de novo gold nanoparticles (Au(0)NPs) that turn on p53 and turn off NF-kB signaling in SKBR3 breast cancer cells. The chemical method was used to make the erythromycin-based Au(0)NPs. Authentic techniques were used to figure out what these Au(0)NPs were like. In the end, relative gene expression studies were used to treat SKBR3 breast cancer cells with these Au(0)NPs as a nanomedicine. When Au(0)NPs were present, the levels of caspases 3, 8, and 9 changed, p53 was turned on, and NF-kB was turned off at the same time. Compared to normal breast cells, the number of breast cancer cells (SKBR3) that could live was cut down (CRL-4010). Gene expressions of caspases also showed that the data were correct. When AuNPs were used to treat breast cancer cells, it was found that p53 and NF-kB had the opposite relationship. The study laid out a first step for using newly made AuNPs as a chemotherapeutic agent to treat SKBR3 cells.