Isolation and Classification of Lipoxygenase from the Rind of Pistacio Khinjuke and Investigation of its Affinity Toward Certain Inhibitors in Mice: in vivo and in vitro

Abstract

This study includes purification oflipoxygenase from the rind of  pistaciokhinjuke by applying ammonium sulfate precipitation (70%), dialysis, ionexchange chromatography, and the slab electrophoresis technique. One isoenzymehas been obtained with a special activity of 1230x10-4 unit/mg protein and a molecularweight of 101997 dalton. The partially purified rind lipoxygenase has beencharacterized and inhibited by quercitine rutinoside and melatonine. The typeof inhibition seemed to be uncompetitive and the Ki values were 1.77 and 1.62mM, respectively. Due to the importance of lipoxygenase for the animal, thisstudy also deals with the determination of its affinity toward quercitinerutinoside and melatonine in the serum of normal and diabetic male mice. Thebest doses for lipoxygenase inhibition were 523.3 and 8 Mg/Kg body weight ofquercitine rutinoside and melatonine, respectively. Treatment of animals withthese doses led to a significant decrease in lipoxygenase activity, glucoselevel, and total cholesterol/high density lipoprotein cholesterol ratio inanimal serum. Therefore, these natural inhibitors seem to serve as a drug forreducing the complications of some diseases (e.g. diabetes mellitus) which areaffected by this enzyme.