Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media

Abstract

Severalexperiments have been conducted to study factors affecting the maturation andfertilization of the cattle ova in the laboratory. Ovaries were collected from77 cow reproductive systems in Al- Shuala Massacre- Baghdad. Oocytes have beenobtained from these samples by OCRC method and finishing. Oocytes wereclassified into several grades from both methods, and then oocytes class A andB only were chosen for maturation, where oocytes were incubated in multiplemedia including; TCM-199, MEM, SOF, SOF+BCS, SOF+BSA in incubator 5% CO2, 90%humidity at 38.5ºC temperature for 24 hours. Semen has been obtained byextracting of the epididymal tail, and then collected by using a sterile 5 mlsyringe containing MEM media. Sperms were incubated with mature oocytes at aconcentration of 1 × 10 6/ml of the cultivated media in the incubator for 30hours for the fertilization and to obtain on zygotes. Zygotes were thenisolated in the growing media. The developing embryos were monitored every 24hours and up to 9 days until the reach blastocyst stage.  Results indicate the possibility of obtainingembryos in vitro fertilization in an average 6.31%  (111/1604), and the best media was by viausing SOF + BCS media. The study also determined appropriate technologies toenhance in vitro fertilization. ORCS method showed a positive effect onincreasing the rate of embryos production in vitro.