Study the Effect of Isolated Osteocalcin from Human and Rats on Lipids Profile in Normal and Alloxan-Induced Diabetic Male Rats

Abstract

This study concerned with an attempts to isolate and purify osteocalcin from healthy human blood plasma and healthy rats serum using different biochemical techniques. This included precipitation by cold acetone and gel filtration chromatography. It was found that only the second peak (peak B) from human and rats had concentration of osteocalcin, and showed that fold of purification of isolated osteocalcin 57 and 43 for human and rats respectively. The research also included study the effect of isolated osteocalcin from human plasma and rats serum on lipids profile in normal and alloxan induced diabetic males rats. The results showed after one week of treatment by the isolated osteocalcin from human plasma and rats serum at the dose (1ng/Kg of body weight/d) caused a significant decrease in the concentration of all from total lipids, total cholesterol, triglycerides, VLDL-C, LDL-C, atherogenic index, and a significant increase in the concentration of HDL-C, and antiatherogenic index in normal and alloxan induced diabetic rats.