Extraction and Purification of the Potential Allergen Proteins from Candida Albicans

Abstract

A commercial strain of Candida albicans10231 was grown and isolated using Sabouraud's Dextrose Agar Media. The samples were then subjected to lyophilization before protein extraction. TCA/acetone/methanol/phenol method has been used for protein extraction. For estimation of the percentage of extracted protein, BCA Protein Macro Assay Kit was applied. The samples were then transferred to the spectrophotometer for reading at wave length of 562 nm. The results show that the profile of the quantity of protein concentrations obtained upon supplementation of the following chemicals: PBS, ammonium bicarbonate, and 50mM of SDS buffer, were 0.133mg/mL, 0.080mg/mL, and 0.068mg/mL respectively.